產(chǎn)品概述
脂聯(lián)素又被稱作Acrp30,AdipoQ,apM1,GBP28, Acrp30是一種分子量為30KD的脂肪細胞補體相關蛋白,基因轉錄物apM1是在脂肪組織中表達最豐富的轉錄物, GBP28是脂肪細胞特異性分泌的28KD凝膠蛋白,在維持機體葡萄糖和脂質平衡上有著重要的作用,脂聯(lián)素結構包括一個膠原樣區(qū)域組成的N-末端和球狀區(qū)域組成的C-末端,此區(qū)域有一段與補體因子C1q非常類似的序列,此外脂聯(lián)素還具有增加抗動脈粥樣硬化和抗炎癥等生物學效應,在機體產(chǎn)生疾病時血漿中的脂聯(lián)素水平會降低。
產(chǎn)品原理
本實驗采用雙抗體夾心ELISA法??谷薃diponectin單抗包被于酶標板上,標本和標準品中的Adiponectin會與單抗結合形成免疫復合物,游離的成分被洗去;加入生物素化的抗人Adiponectin抗體,生物素化抗人Adiponectin抗體與酶標板上結合的標準品或樣本中的Adiponectin結合而形成免疫復合物,游離的成分被洗去;加入辣根過氧化物酶標記的親合素,親合素與生物素特異性結合,游離的成分被洗去。加入顯色底物(顯色劑),若反應孔中有Adiponectin,辣根過氧化物酶會使無色的顯色劑變藍,加終止液變黃。在450nm處測OD值,Adiponectin濃度與OD450值之間呈正相關,可通過繪制標準曲線求出標本中Adiponectin濃度。
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